By John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar, Michael M. Gottesman
The severely acclaimed laboratory average for greater than 40 years, Methods in Enzymology is among the so much hugely revered guides within the box of biochemistry. seeing that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. greater than 285 volumes were released (all of them nonetheless in print) and masses of the cloth is appropriate even today-truly an important ebook for researchersin all fields of existence sciences.
* Prokaryotic ABC Transporters
* Eukaryotic ABC Transporters
* Nonmammalian ABC shipping Systems
* Mammalian P-Glycoproteins
* Multidrug Resistance linked Protein
* Cystic Fibrosis Transmembrane Conductance Regulator
* Sulfonylurea Receptor
* Intracellular ABC Transporters
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Extra info for ABC Transporters: Biochemical, Cellular, and Molecular Aspects
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Ling, Methods Enzymol. 292, , 1998 (this volume). 2 R. Binet, S. Letoffe, J. Ghigo, P. Delepelaire, and C. W a n d e r s m a n , Gene 192, 7 (1997). 3 W. Boos and J. M. Lucht, in "Escherichia coli and Salmonella'" (F. C. , p. 1175. A S M Press, Washington, DC, 1996. 4 H. W i e s m e y e r and M. Cohn, Biochim. Biophys. Acta 39, 440 (1960). s W. Boos, Methods" EnzymoL 292, , 1998 (this volume). T. J. Silhavy, E. Brickman, P. J. Bassford, M. J. Casadaban, H. A. Shuman, V. Schwartz, L. Guarente, M.
USA 89, 2360 (1992). 13 A. L. Davidson, S. S. Laghaeian, and D. E. Mannering, J. Biol. Chem. 271, 4858 (1996), 14 M. Reyes and H. A. Shuman, J. Bacteriol. 170, 4598 (1988).  MALTOSE TRANSPORTER OF E. coli 23 Preparation of Membrane Vesicles Membrane vesicles are prepared as follows. All procedures are carried out at 4° unless stated otherwise. 0, 1 mM EDTA, 1 mM phenylmethylsulfonylfluoride (PMSF), 50/xg/ml DNase, 50/xg/ml RNase A and passed through a precooled French pressure cell at 12,000 psi twice.
ABC Transporters: Biochemical, Cellular, and Molecular Aspects by John N. Abelson, Melvin I. Simon, Suresh V. Ambudkar, Michael M. Gottesman