By Stewart Martin, Cliff Murray
A entire choice of key concepts for assessing the formation of recent blood vessels in either complete tissues and telephone tradition. those simply reproducible equipment variety from inexpensive in vitro assays to state-of-the-art in vivo versions, comparable to the dorsal window chamber, which calls for excessive degrees of surgical ability in addition to rather pricey, personalized apparatus. well timed and hugely sensible, Angiogenesis Protocols is a gold-standard source for ultra-modern angiogenesis investigator, delivering easy accessibility to a wide selection of confirmed assays compatible for laboratories with drastically differing technical services, fabrics, and fiscal assets.
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Guidi, A. , Dvorak, H. , et al. (1995) Expression of vascular permeability factor (vascular endothelial growth factor) and its receptors in breast cancer. Hum. Pathol. 26, 86–-91. , Zhang, H. , Fan, T. , Hu, D. , Lees, V. , et al. (1995) Thymidine phosphorylase is angiogenic and promotes tumor growth. Proc. Natl. Acad. Sci. USA 92, 998–1002. Anandappa, S. , Winstanley, J. , Rudland, P. , and Barraclough, R. (1994) Comparative expression of fibroblast growth factor mRNAs in benign and malignant breast disease.
5. If sections continually float off after antigen retrieval, drying at 56°C overnight will increase tissue adherence. 6. Alternative antigen retrieval techniques include pressure cooking and microwaving. 7. APAAP is preferred in tissues such as liver and kidney, which contain high endogenous biotin. 8. This can be obtained from Graticules Ltd, Morely Road, Botany Trading Estate, Tonbridge Wells, Kent, TN9 1ZN, UK. NB. Graticule size will depend on the microscope eyepiece diameter. 9. The field area would be too small were a higher magnification to be used.
1992) Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J. Natl. Cancer Inst. 84, 1875–1887. , Lee, A. , DeLellis, R. , Wiley, B. , Heatley, G. , Silverman, M. L. (1992) Microvessel quantitation and prognosis in invasive breast carcinoma. Hum. Pathol. 23, 755–761. , and Knox, F. (1994) Angiogenesis in breast cancer predicts node metastasis and survival. Br. J. Surgery 81, 768 (abstract). , et al. (1994) Microvessel count and cerebrospinal fluid basic fibroblast growth factor in children with brain tumors.
Angiogenesis Protocols (Methods in Molecular Medicine) by Stewart Martin, Cliff Murray