By Joseph Sherma, Bernard Fried
Written by means of over forty across the world acclaimed professionals on thin-layer chromatography (TLC), this finished moment version provides the most recent options, instrumentation, and purposes of overpressurized, rotational, and high-performance quantitative TLC.
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Written by way of over forty the world over acclaimed specialists on thin-layer chromatography (TLC), this accomplished moment version offers the newest innovations, instrumentation, and functions of overpressurized, rotational, and high-performance quantitative TLC.
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Extra resources for Handbook of Thin-Layer Chromatography
The zone elution quantification method is tedious and time consuming and is likely to be inaccurate because of difficulties in locating the exact zone boundaries, loss of sorbent during scraping and collection, nonreproducible or incomplete elution from the sorbent, and background interferences due to eluted impurities from the sorbent. These errors are minimized if standards and samples are chromatographed, scraped, and eluted together as consistently as possible, and if an equal-size blank Sherma 36 area of layer is scraped and eluted in the same way.
Samples for preparative layer chromatography are applied as bands across thicker layers. This can be done manually by allowing sample to flow from a pipet drawn along the edge of a ruler. The Camag Linomat, described above, is among the special instruments designed to apply samples more uniformly. Analtech Preparative Uniplate-T wedge-shaped layers are tapered from a thickness of 1700 ц т at the top to 300 |im at the bottom, and have a 700 (im-thick preadsorbent area at the bottom for manual or instrumental sample application.
B. Zone Elution The zone elution method involves the following steps: drying the layer, locating the separated analyte zone, scraping the portion of layer containing the analyte, collection of the sorbent, elution of the analyte from the sorbent, and measurement against standards by an independent microanalytical method such as solution absorption or fluorescence spectrometry, GC, HPLC, or voltammetry. The chromatogram is dried to remove the mobile phase, components of which might serve as interferences in the determinative step.
Handbook of Thin-Layer Chromatography by Joseph Sherma, Bernard Fried